Biological safety cabinet (BSC) or a Biosafety Cabinet is a ventilated containment device found in laboratories around the world. BSCs are used in many applications, including cell culture, pharmaceutical, clinical and microbiological work. These work-spaces help protect the user and surrounding environment from pathogens. Engineered controls are built into the design of BSCs providing protection to the operator, product and environment. All exhaust air is HEPA filtered to remove hazardous agents such as viruses and bacteria. Many laboratories including clinical and research labs use biosafety cabinets.
There are three classes of biosafety cabinets available. Those are named Class I, II and III. Class I provides protection for the user and surrounding environment, but no protection for the sample being manipulated. Class II provides protection for the user, environment and sample, and is divided into four types: A1, A2, B1 and B2. The main differences are their minimum inflow velocities and exhaust systems. Class III, also known as glove boxes, gives the maximum protection; the enclosure is gas-tight, and all materials enter and leave through a dunk tank or double-door autoclave.
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